N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester
- Product Name
- N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester
- CAS No.
- 1345980-40-6
- Chemical Name
- N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester
- Synonyms
- Fluo-2 AM;Fluo-8, AM;Calcium Fluorescent Probe Fluo-8, AM;N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester;Glycine, N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]-, (acetyloxy)methyl ester
- CBNumber
- CB32755640
- Molecular Formula
- C50H50N2O23
- Formula Weight
- 1046.93
- MOL File
- 1345980-40-6.mol
N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester Property
- Boiling point:
- 1051.0±65.0 °C(Predicted)
- Density
- 1.45±0.1 g/cm3(Predicted)
- storage temp.
- -20°C
- solubility
- Soluble in DMSO (up to 25 mg/ml).
- form
- waxy solid
- pka
- 1.56±0.50(Predicted)
- color
- Dark
- Stability:
- Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
- Precautionary statements
-
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P321Specific treatment (see … on this label).
P332+P313IF SKIN irritation occurs: Get medical advice/attention.
P362Take off contaminated clothing and wash before reuse.
N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester Chemical Properties,Usage,Production
Description
FLUO-8 (AM) is a cell-permeable acetoxymethyl (AM) ester derivative of Fluo-8. Approximately twice as bright as Fluo-4. Absorbance 452 nm (26,000); Emission 515 nm (after AM hydrolysis; dissolved in 10 mM EGTA, 100 mM KCl, 10 mM MOPS, pH 7.2)
Uses
FLUO-8 (AM) is a green fluorescent calcium binding dye. It is a tool in making the determination of drug resistance in cells.
References
1) Minta et al. (1989), Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores; J. Biol. Chem., 264 8171